VIETNAMESE GINSENG EXTRACT ATTENUATES OXIDATIVE STRESS IN COBALT CHLORIDE-SUBJECTED H9C2 CELLS

Abstract

Objective: This study aimed to evaluate the protective effects of Vietnamese ginseng extract
(VGE) on the formation of reactive oxygen species (ROS) in Cobalt chloride (CoCl2)-exposed
H9C2 cells. Methods: H9C2 cells were subjected to CoCl -induced hypoxia-reoxygenation (HR)
model with or without treatment of VGE. The hydroperoxide (H2O2) and superoxide (O2
-)
productions in H9C2 cells in the experimental groups were assessed by suitable fluorescence
kits. Results: The obtained data showed that VGE at a dose of 31.25 μg/ml significantly reduced
ROS levels in HR-subjected H9C2 cells. In the HR group, the H2O2 and O2
- content in H9C2 cells
were sharply increased to 156.81±7.82% and 160.10±2.07% (of 100% control), respectively.
Interestingly, in the VGE-treated HR group, these indicators were significantly decreased to
119.67± 3.37% and 124.72 ± 3.21% (p <0.05), respectively. Conclusion: The results
demonstrated that VGE effectively attenuated oxidative stress in cardiomyocytes under CoCl2-
induced HR condition.