HOẠT TÍNH KHÁNG VIÊM CỦA CAO CHIẾT SÂM VIỆT NAM (PANAX VIETNAMENSIS) ĐỐI VỚI TẾ BÀO RAW 264.7

Abstract

Panax vietnamensis (SVN) is a precious and endemic medicinal plant of Vietnam. SVN has been reported to have immune-boosting, antioxidant, and anti-cancer effects. However, the effect of this ginseng on inflammatory lesions has not been elucidated yet. This study was conducted to evaluate the anti-inflammatory activity of the aqueous and butanol extracts of SVN on LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were cultured under either normal environmental conditions or treated with LPS. The extracts of SVN was added prior to LPS treatment. At the end of the experimental period, cell viability, nitric oxide (NO) and reactive oxygen species (ROS) indexes were determined by using CCK-8, Griess Reagent and CM- H2DCFDA kit. The obtained results showed that the butanol extract (50 and 100 µg/ml) markedly increased the cell survival rate of RAW 264.7 cells under LPS treatment. The LPS-stimulated RAW 264.7 cells supplemented with butanol extract had a significant reduction in NO and ROS compared to the non-supplemented cell group. However, the aqueous extract of SVN did not show these activities. The findings provide preliminary evidence that the butanol extract of SVN has a protective effect on RAW 246.7 cells via reducing inflammatory mediators, such as NO and ROS.