TÌNH TRẠNG TĂNG ĐƯỜNG HUYẾT VÀ RỐI LOẠN LIPID MÁU TRÊN MÔ HÌNH CHUỘT NHẮT TRẮNG ĐỰC GÂY BÉO PHÌ BẰNG THỨC ĂN GIÀU LIPID

Abstract

Objective: The study was conducted to evaluate hyperglycemia and dyslipidemia in mice that were generated obesity model. Methods: Male mice (Swiss albino) were divided into 2 groups (12 animals per group), including the NFD group (Normal-fat diet) and the HFD group (High-fat diet). Mice were weighed weekly. After 6 weeks, mice were evaluated for BMI (Body mass index), performed OGTT (Oral glucose tolerance test) at 4-time points (0 hours, 0.5 hours, 1 hour, 2 hours), and measured serum levels of lipid profile (total cholesterol, triglyceride, HDL-c, LDL-c) by Cobas C311 biochemical analyzer. Results: After 6 weeks of feeding by a high-fat diet, mice of the HFD group gained 176.57±21.68% in weight (131,32% higher than that of the NFD group), with BMI = 5.66±0.30kg/m². The difference in mean weight of the 2 study groups started to appear clearly from the end of week 2 (p<0.001). Fasting blood glucose levels and blood glucose levels at 3-time points after OGTT of the HFD mice were statistically significantly higher than that of NFD mice (p<0.001). Therefore, the level of glycemic tolerance of the HFD group was worse than that of the NFD group. In addition, the total serum cholesterol, triglyceride, HDL-c, and LDL-c levels of the HFD group were statistically significantly higher than that of the NFD group (p<0.01). Conclusion: Obesity mice fed with a high-fat diet had hyperglycemia, impaired glucose tolerance, and dyslipidemia after 6 weeks of modeling.