TÁC DỤNG BẢO VỆ TẾ BÀO HEK293 CỦA RUTIN TRONG ĐIỀU KIỆN THIẾU ÔXI/TÁI CUNG CẤP ÔXI IN VITRO SỬ DỤNG BUỒNG WHITLEY H35

Abstract

Objectives: This study was conducted to evaluate to evaluate the effectiveness of in vitro hypoxia-reoxygenation (HR) model using a Whitley H35 Hypoxystation chamber and initially determine the effect of Rutin (Rut) on HEK293 renal epithelial cells. Methods: The effectiveness of the HR model and the role of Rut were determined by evaluating the percentage of viable cells using the CCK-8 kit. Results: The HR model using the hypoxia chamber Whitley H35 Hypoxystation has a hypoxia period of 6 hours (0.5% O₂, 37⁰C, 5% CO₂) and a 24-hours reoxygenation interval showing the efficiency when using HEK293 renal epithelial cells as study subjects. Rut is less cytotoxic at concentrations lower than 250 µM; especially, Rut at concentrations of 10, 20 and 40 µM exhibited protective effect on HEK293 cells in HR-induced injury. The data initially shows the potential role of Rutin in protecting HEK293 cells again HR injury.