DETECTION AND QUANTIFICATION OF GROWTH FACTORS IN UMBILICAL CORD MESENCHYMAL STEM CELL-DERIVED EXOSOMES AND UMBILICAL CORD BLOOD-ORIGNATED PLATELET RICH PLASMA

Abstract

Objective: Describing the expression of several growth factors in exosomes released by
umbilical cord-derived mesenchymal stem cells (UCMSCs) and platelet rich plasma (PRP)
derived from umbilical cord blood (UCB). Methods: 05 UCMSC samples were cultured for the
release of exosomes into conditioned media. 05 UCB-dereived plasma samples were used to
prepare PRP. Conditioned media was harvested from UCMSCs at passage four (P4) was
differentially centrifuged for exosomes. Isolated exosomes and PRP were then investigated for
the expression of growth factors. The growth factors which are from exosomes and PRP include:
hepatocellular growth factor (HGF), platelet-derived growth factor BB (PDGF-BB), vascular
endothelial growth factor A (VEGF-A), and fibroblast growth factor 2 (FGF-2). Results: Both
UCMSC-derived exosomes and UCB-derived PRP carried growth factors with different amount.
The expression of HGF (2176.82 pg), PDGF-BB (1765.82 pg) and VEGF-A (2336.43 pg) is much
greater than FGF-2 (39.64 pg) and HGF (2176.82 pg) is larger than PDGF-BB (1765.82 pg) in
the UCB-derived PRP. Regarding UCMSC-derived exosomes, HFG (312.62 pg) and PDGF-BB
(674.87 pg) were expressed larger than VEGF-A (72.50 pg) and FGF-2 (34.51 pg). Conclusion:
Both UCMSC-derived exosomes and UCB-derived PRP contained abundant amount of growth
factors, including HGF, PDGF-BB, VEGF-A, and FGF-2. Expression level of each factor is
different and the FGF-2 is the lowest expression in both exosomes and PRP.