ĐIỀU KIỆN LY TÂM TỐI ƯU CHO QUÁ TRÌNH SẢN XUẤT HUYẾT TƯƠNG GIÀU TIỂU CẦU

Abstract

Background:Platelet-rich plasma (PRP) is now applied efficiently in some clinical | scenarios. Centrifugation is one of the most important steps in preparing platetlet-rich : plasma (PRP), but there remains arguments about the optimal centrifugal condition, i which include the relative centrifugal force and time period of centrifugation. Objectives: This study aims to figure out the best conditions for preparing PRP from venous blood. Subject and methods:The collected samples of blood from healthy donors were centrifuged at different centrifugal forces (16g, 44xg, 87* d 177xg) in i different time (5, 10, 1 5 minutes). The total blood ceil EBiini ie _ ca d in whole i blood and PRP to evaluate the affection of different centrift ` aie ' lood h samples. Results: Our method showed four optimal conc ugal BGIETHIBHS on b : 2 platelet concentration are centrifugations of 16xg in 15 mi ns for obtaining the hig độ | min and 177xg in 5 min, in which the centrifugation of 44 = 44xg in 10 min, 87xg in d yield the maximum recovery of platelet. Conclusi +xg in 10 min was determined to conditions above to prepare PRP for osteoarthritis t on: We can use one of the four centrifugal acceleration and time of 10 min is the b ig Satment, and the setting of 44*9 for