OLEANOIC ACID ALLEVIATES BONE DAMAGE IN A MEDAKA OSTEOPOROSIS MODEL

Abstract

Objective: To evaluate bone antiresorptive effect of oleanoic acid (OA) in the rankl:HSE:CFP
transgenic medaka (Oryzias latipes) fish model for osteoporosis. Method: Transgenic larvae
were treated with OA at different concentrations from 7 days post fertiliztion (dpf) onwards,
heatshocked at 9 dpf to induce osteoporosis-like phenotype and fixed at 11 dpf to stained for
mineralized bone matrix. Length of the first 15 neural arches were measured and indexes of bone
mineralization (IM) and indexes of mineralization protection (IP) were calculated to evaluate bone
protective effect of OA. Results: Mean IM of larvae treated with OA at concentration of 7.5 μM,
12.5 μM, 15 μM or 20 μM was significantly higher than that of the DMSO control larvae and similar
to that of alendronate-treated larvae. Moreover, 15 μM or 20 μM OA has higher IP than 25 μg/ml
alendronate, a common anti-osteoporosis drug. Conclusion: Oleanoic acid has bone protective
effect in the Rankl-induced osteoporosis medaka and this bone protective effect is comparable to
alendronate.