NGHIÊN CỨU TÁC DỤNG CỦA CAO SÂM VIỆT NAM (PANAX VIETNAMENSIS) ĐỐI VỚI TẾ BÀO CƠ TIM H9C2 TRONG MÔ HÌNH THIẾU OXY-TÁI CUNG CẤP OXY IN VITRO

Abstract

Objective: Vietnamese Ginseng (Panax vietnamensis, SVN) or Sam Ngoc Linh is a potential herbal drug and is widely used in Viet Nam. However, the effects of SVN on cardiomyocytes in hypoxia-reperfusion injury have not been elucidated yet. This study aimed to evaluate the protective effects of SVN extract on H9C2 cardiomyocytes in CoCl2-induced hypoxia-reperfusion model in vitro. Methods: H9C2 cells were cultured under normal (control) and subjected to HR conditions. The cell viability, the cardiolipin contents and the mitochondrial membrane potential were analyzed. Results: The results showed that, SVN extract at doses of 10÷500 µg/ml significantly increased the survival rate of H9C2 cells against HR. Especially at the dose of 31.25 µg/ml, SVN extract strongly preserved the cardiolipin contents and the mitochondrial membrane potential of H9C2 cells under HR condition. Conclusion: These obtained results demonstrated that SVN extract effectively protected cardiomyocytes agaist HR injury targeting mitochondria.