BƯỚC ĐẦU XÂY DỰNG QUY TRÌNH XÁC ĐỊNH CHUỖI NHẸ TỰ DO KAPPA VÀ LAMDA TRONG HUYẾT TƯƠNG VÀ DỊCH NÃO TUỶ CỦA BỆNH NHÂN VIÊM NÃO TỰ MIỄN

Abstract

Objectives: To evaluate the possibility to determine the presence of the free light chains (FLCs) in autoimmune encephalitis, the study utilize Western Blot to detect and semi-quantify the kappa and lamda free light chains in the cerebrospinal fluid and plasma of patients. Subjects: 9 pairs of plasma and cerebrospinal fluid samples from 9 patients diagnosed with autoimmune encephalitis provided by the National Children's Hospital. Methods: Albumin and IgG were fractionated by affinity chromatography and separated by 2-D electrophoresis. The kappa and lamda FLCs were determined by Western Blot, then quantified using ImageJ software. Results: Albumin and IgG are the two major components of plasma, they consist of many isotypes, separated as spots on 2D electrophoresis gel. The expression of kappa free light chain was obviously observed in all 9 CSF and plasma samples. None of lamda free light chains were recorded in the patient's CSF. Conclusions: The kappa free light chain is strongly expressed in CSF samples and Western Blot is a potential method that can be developed as an adjunct to the diagnosis of autoimmune encephalitis.

Keywords: Free light chains, autoimmune encephalitis, multiple sclerosis, cerebrospinal fluid.