Tính ổn định của gen chuyển RANKL ở dòng cá MEDAKA chuyển gen RANKL:CFP làm mô hình bệnh loãng xương

Abstract

transgenic medaka rankl:HSE:CEP fish expressing both Rankl and CFP after being heat-shocked was generated in the National University of Singapore. In this fish, induced over-expression of Rankl promotes osteoclastogenesis, leading to bone demineralization and osteoporosis-like Phenotypes. This fish line was kindly provided to our laboratory at the Faculty of Biology, VNU University of Science for use as a model for osteoporosis and screening for anti-osteoporosis substances. However, the use of medaka as a model organism has been largely unknown_in Vietnam. Therefore we established fish facility and procedures to maintain medaka fish in our laboratory. Moreover, we have examined and confirmed that the transgene rankl is genetically and functionally stable in the rankl:HSE:CFP fish after four generations maintained in our facility. This assures the use of the transgenic rankl:HSE:CFP medaka as a model for osteoporosis for further studies. ;